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Fig. S2
Tbx4/5 and Flt1/4 expression in sea lamprey embryos. Tbx4/5 (AL) and Flt1/4 (MP) expression in developing sea lampreys. (A) Tbx4/5 was undetectable at stage 21. (B) Tbx4/5 is initially detected in the heart primordium at stage 22. (CF) Tbx4/5 is expressed in the heart. The posterior border of Tbx4/5 expression and heart domain are not clear due to the premature development of heart and cardinal veins at these stages. (G and H) The heart expression of Tbx4/5 becomes barely detectable at stages 27 and 28. (I-L) Tbx4/5 is undetectable in the heart at stages 29 and 30. The expression is never found in the median fin (J and L). Tbx4/5 expression was not found in the dorsal portion of the eye in sea lamprey embryos. (M) Flt1/4 was detectable in the dorsal and ventral aortas, but not in the cardinal veins at stage 23. (N and O) Flt1/4 was detected in the cardinal veins at stages 24 and 25. (P) Flt1/4 was weakly expressed in the cardinal veins at stage 26. (Scale bars: 200 µm.)