Fig. 4
Development of bath infection model. (A) Larvae were injured in the tail fin tip using a sapphire blade. (B) Cumulative mortality of healthy and injured larvae infected with A. hydrophila (108 CFU/mL). Fish in the control group were injured in the tail fin and treated with PBS. Graph is a representative result of five independent experiments using 40 larvae in each experiment. The injury induced a significant increase of mortality (*P < 0.05). (C) Bacterial burden in infected healthy and injured larvae at 1, 6, and 24 hpi. Data represent the mean ± SEM of two independent experiments (n = 40 larvae/each). Significant differences at **P < 0.01.