Fig. 2
Neutrophils are indispensible for zebrafish survival from A. baumannii infection. (A) Phagocytosed A. baumannii (pHrodo-dextran stain, white arrow) is handled within acidic phagolysosomes within a neutrophilic vacuole at 50-min postinfection [Tg(mpx:GFP)il14 embryos with green fluorescent neutrophils were used]. (Scale bar: 20 µm.) Survival of neutrophil-depleted embryos (csf3r MO) (B) or macrophage-depleted embryos (irf8 MO) (C) after bloodstream infection with A. baumannii. Comparison is made vs. A. baumannii-infected embryos injected with control morpholino (Ctrl MO; P < 0.001; n = 30 embryos, three biological replicates).