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Fig. S1
Cortisol treated larvae have higher levels of Glucocorticoid Receptor activity. (A) Expression of known glucocorticoid-responsive genes fkbp5, tsc22d3, and mmp13 measured by qRT-PCR and RNASeq untreated control larvae (-) and larvae treated with 1 µM cortisol (+) from 0-5 days post-fertilization. Individual data points represent measurements from one of three biological replicates. Error bars represent standard deviation of three technical replicates for each measurement. Differences in average expression between control and treatment groups are all statistically significant (p < 0.05). (B) Fluorescence in untreated and cortisol-treated SR4G larvae. The bright fluorescence in the yolk sac is autofluorescence, which was excluded from the measurements shown in Fig. 1C.