Fig. 1

Fig. 1

A small 195 bp unc45b promoter fragment drives cardiac and skeletal muscle expression. (A) Schematic representation of the full-length unc45b promoter 1.8(unc45b:tfp), a 195 bp unc45b promoter fragment associated with gata2 (unc45b^mingata2:gfp) and the 195 bp unc45b promoter fragment alone (unc45b^min:gfp). (B) Injection of unc45b^mingata2:gfp into wild-type embryos reveals GFP expression within cardiac and skeletal muscle (72 hpf). (C) Transgenic unc45b^mingata2:gfp embryos Tg(unc45b^mingata2:gfp) show a restricted expression in skeletal and cardiac muscles at 72 hpf. (D) Microinjection of unc45b^min:tfp into wild-type embryos also reveals a restricted expression in skeletal and cardiac muscles (72 hpf). (E) Tg(unc45b^min:gfp) transgenic zebrafish embryos show heart- and skeletal muscle-specific GFP expression (72 hpf). (F, G) Heat shock significantly increases GFP levels in Tg(unc45b^min:gfp) embryos (G) compared to untreated embryos (F) at 72 hpf.