ZFIN Image: Doll et al., 2011, Fig. S1

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Figure Caption

Fig. S1

The c163 lesion is a nonsense mutation in sec61al1. (A) Linkage to microsatellite markers was used to map c163 close to z7248, near the sec61al1 locus. (B) c163 mutants are characterized by a reduction in jaw and head volume, and a short, dorsally curved axis. These phenotypes are also seen in embryos injected with 500 pg sec61al1^ATG-MO. (C) Compared to a non-injected control, (C′) treatment with a morpholino complementary to the start site or (C′′) to a splice acceptor site in the sec61al1 transcript results in excess kctd12.1-expressing neurons in the right habenula. (D, D′) The retroviral insertion mutant, sec61al1^hi1058 fails to complement c163. Transheterozygotes for hi1058 and c163 have increased numbers of kctd12.1-expressing cells in the habenulae. Scale bars: B-D′ = 20 µm.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 360(1), Doll, C.A., Burkart, J.T., Hope, K.D., Halpern, M.E., and Gamse, J.T., Subnuclear development of the zebrafish habenular nuclei requires ER translocon function, 44-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.