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Fig. 5
Formation of the horizontal myoseptum is affected in doctt258 embryos. (A) Somites of a wild-type embryo showing the characteristic vshape at 24 hours of development. (B) In doctt258 embryos, as in flh, mom and ntl embryos, the somites are u-shaped instead of v-shaped. (C) In the apex of the v, the nuclei of muscle pioneer cells stain with the antibody against Eng. (D) Eng staining in the somites is not detectable in doctt258 embryos, whereas expression in the midbrain-hindbrain boundary is normal (not shown). Lateral views, anterior is to the left.
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