ZFIN Image: Bryan et al., 2016, Fig. 1

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Fig. 1

Timelapse confocal microscopy reveals severe defects in optic cup formation in lama1^UW1 mutant. (A-H) Single confocal slices from 4D datasets of optic vesicle morphogenesis, 12.5-24.5 hpf. EGFP-CAAX (membranes, green), H2A.F/Z-mCherry (nuclei, magenta). (A-D) Optic cup formation in control embryo. (E-H) Optic cup formation in lama1^UW1 mutant embryo. Dorsal views; scale bar, 50 µm. (I-M) Localization of laminin protein. (I-L) In control embryos, laminin protein is found lining basal surfaces of developing eye and brain. (M) lama1^UW1 mutant embryo reveals absence of laminin protein from early stages of optic vesicle development. nk, neural keel; ov, optic vesicle; ec, ectoderm; br, brain; RPE, retinal pigmented epithelium; nr, neural retina; le, lens. A, anterior; P, posterior; M, medial; L, lateral.

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Acknowledgments

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Reprinted from Developmental Biology, 416(2), Bryan, C.D., Chien, C.B., Kwan, K.M., Loss of laminin alpha 1 results in multiple structural defects and divergent effects on adhesion during vertebrate optic cup morphogenesis, 324-37, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.