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Fig. S6
Enlarged early endosomes grow and accumulate over time in NC cells of rbc3a-MO1-injected embryos. Confocal images of sox10:gfp+ NC cells (bottom row, green) double labeled with anti-EEA1 (A-F, red) and anti-Rab11a (A′-F′, blue), which marks late endosomes. (A′′-F′′) Merged images of EEA1, Rab11a, and sox10:gfp fluorescence. EEA1+ vesicles increase in number and size in rbc3a-MO1-injected embryos from 14-20 hpf compared to wild-type controls, while Rab11a+ vesicles show no change. Scale bars, 10 µm.
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