ZFIN Image: Yabe et al., 2016, Fig. S3

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Figure Caption

Fig. S3

Generation of ripply1 and ripply2 double mutant using TALENs. (A) Schematic diagram showing mutations of ripply1 and ripply2. Colored boxes indicate the coding regions of Ripply1 and Ripply2 proteins; green boxes indicate the Ripply-homology domain, which is required for physical interaction with Tbx6, and blue boxes indicate the WRPW motif, which is essential for the interaction with Groucho. Red arrowheads indicate approximate positions of the mutations. The DNA sequences around the mutation sites are given below the schematic diagrams. Red bars indicate the mutated sequence. (B, C) The morphologies of rippley1;ripply2 double mutant embryo at the 13-somites stage.

Acknowledgments

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