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Fig. 4 Results from Whole-Cell Voltage-Clamp Recordings from WT and re[a(Δ10)/b(Δ7)] Homozygous Mutant Neuromast Hair Cells
(A) Average whole-cell capacitance measurements of WT (black, n = 11) and re[a(Δ10)/b(Δ7)] homozygous mutants (red, n = 12) neuromast hair cells in response to 3-s step-depolarizations. Note that all traces include error bars denoting SEM for each condition. The average capacitance increase at the end of the 3-s depolarization (averaged over 100 ms) was 67.8 ± 5.0 fF for the WT hair cells and was 106.1 ± 13.6 fF in the mutants. p = 0.018.
(B) Averaged calcium current trace of WT (black) and re[a(Δ10)/b(Δ7)] homozygous (red) mutants in response to a 20-mV step depolarization.
(C) Plot of current-voltage relationship for WT fish NM hair cells (black, n = 15) and re[a(Δ10)/b(Δ7)] homozygous mutant neuromast hair cells (red, n = 17). At 20 mV, the average current in WT cells was 6.1 ± 0.5 pA, and mutant fish cells was 8.3 ± 0.6 pA. p = 0.010. The fish recorded were between 5 and 8 days old, and the neuromasts recorded were P3 and P4.
(D) Capacitance recording of WT fish at 2.8 mM Ca2+ (black) and 10 mM Ca2+ (blue) external solution and double-mutant fish at 2.8 mM Ca2+ external solution (red) in response to a 3-s step depolarization. The average capacitance increase at the end of the 3-s depolarization (averaged over 100 ms) was 105.09 ± 13.74 fF for the WT hair cells at 10 mM Ca2+ solution. Capacitance increases in WT and double-mutant fish in 2.8 Ca2+ external solution are the same as in (A).
(E) Plot of current-voltage relationship of WT fish at 2.8 mM Ca2+ (black) and 10 mM Ca2+ (blue) external solution, and double-mutant fish at 2.8 mM Ca2+ external solution (red). At 20 mV, the calcium current of WT fish in 10 mM Ca2+ external solution is 9.39 ± 1.40 pA, compared to double-mutant fish in 2.8 mM Ca2+ external solution is 8.26 ± 0.58 pA.