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Fig. S5
(A) Western blot analysis of LC3 conversion in RAJI cells treated with 15 µM Iso-3 for 12 h. Where indicated, bafilomycin A1 (40 nM) was added 2 h before harvesting. Cells treated 4 h with PP242 (10 µM) were used as a positive control for autophagy induction. Blot is representative of three independent experiments. (B) RAJI control cells exhibit low basal autophagy levels. (1) Lysosomes, (2) autolysosomes. A RAJI cell treated with 15 µM Iso-3 for 24 h shows a large autophagocytic vacuole (upper image); in extreme cases, autophagy induction culminates in autophagic cell death, evidenced by extensive vacuolization, organelle depletion while nucleus is non-pyknotic (lower image). (3) A large autophagocytic vacuole with membrane remnants.