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Fig. 4
Knockdown of MYO9A in zebrafish. (A) RT-PCR confirming knockdown success of myo9aa and myo9ab in zebrafish injected with morpholino oligonucleotide. There is loss of the myo9aa transcript and an alternatively spliced myo9ab transcript produced due to intron retention. Bands from the same gel have been cropped and put together for comparison, demarcated by the white lines. (B) Images of non-injected and mild/moderate myo9aa/ab morpholino oligonucleotide-injected zebrafish (48 hpf), with the latter displaying varying severities of tail curvature. Scale bar = 100 µm.
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