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Fig. 7
Suppression of non-neuronal expression using synthetic 3′ untranslated regions. (A) Development of synthetic utr-zb3 for suppressing Gal4 reporter expression in muscle, skin and heart. We used Gal4 enhancer trap lines y323Et, y304Et, and y376Et to drive expression in muscle (bracket), heart (asterisk), and skin (arrowheads) respectively-each of these lines also drives expression in the brain. The full pattern of expression is seen using a UAS:epNTR-TagRFPT reporter which has a standard ocean pout anti-freeze protein (afp) 3′ UTR (left panels). Replacing the afp with utr-zb1 eliminates muscle but not heart or skin expression. Similarly utr-zb2 additionally eliminates cardiac expression and utr-zb3 suppresses expression in muscle, heart and skin. (B) Schematic of the 3′ untranslated region in utr-zb3. MicroRNA sites are placed at the beginning of the 3′ UTR, ahead of the afp sequence.