Fig. 6
Nr2e3 inhibits specification of parapineal neurons and is necessary for pineal rod photoreceptors specification. (A) Representative images of antibody labeling of Nr2e3, Rhodopsin and krt4:eGFP in the pineal complexes of non-injected controls (NIC) and nr2e3 splice morphants at the indicated stages. Dorsal views. Scale bars: 30µm. (B) Quantification of the number of Rhodopsin- or Nr2e3-positive cells at 2dpf in NICs, nr2e3 splice morphants, nr2e3 mRNA-injected or nr2e3 morphants/nr2e3 mRNA-injected embryos; mean±s.e.m. and number of samples (n) are shown. ****P<0.0001, P>0.05 (n.s.), one-way ANOVA with Dunnett′s post-hoc analysis.