ZFIN Image: Khuansuwan et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Mosaic overexpression of Tbx2b does not induce additional parapineal neurons; mosaic overexpression of Flh or Nr2e3 suppresses the number of parapineal neurons. (A) Representative images of 2dpf antibody-labeled pineal complexes (foxd3:GFP+) with the indicated protein mosaically overexpressed. Scale bar: 30µm. (B) Quantification of the number of parapineal neurons in Tg[foxd3:GFP] ^zf10; Tg[cfos:gal4vp16]^s1145t double transgenic embryos injected with pDestTol2CG2(4nrUAS:tagRFP-T:polyA) (tRFP), pDestTol2CG2(4nrUAS:tagRFP-T:tbx2b) (tRFP:Tbx2b), pDestTol2CG2(4nrUAS:flh:tagRFP-T) (Flh:tRFP), or pDestTol2CG2(4nrUAS:tagRFP-T:nr2e3) (tRFP:Nr2e3); mean±s.e.m. and number of samples (n) are shown. ***P <0.001, *P<0.05, P>0.05 (n.s.), one-way ANOVA with Dunnett′s post-hoc analysis, comparisons with tRFP shown.

Acknowledgments

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