ZFIN Image: Sako et al., 2016, Fig. S1

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Figure Caption

Fig. S1

Embryo light exposure and subcellular localization of Opto-�acvr 1b and 2b. (A-D) Tg(gsc:GFP) embryos at bud stage (10 hpf) either kept in the dark (A, C) or exposed to blue LED light (B, D; 5.12 �W/mm2, see material and methods) from 2-�cell to bud stage (0.5�10 hpf); upper (A, B) and lower (C, D) panels show bright-field and fluorescent images, respectively; scale bar, 200 �m. (E�H) Anti- HA (Opto-acvr 1b-� or 2b�HA), phalloidin (filamentous actin), and DAPI (nuclei) staining of cells at the animal pole of opto�acvr 1b or 2b mRNA (20 pg each)-� injected MZoep embryos at high stage (3 hpf); scale bar, 20 �m.

Acknowledgments

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