ZFIN Image: George et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Maturation of autophagosomes is blocked in nrc^a14 cones. Expression of the tandem mCherry-GFP-LC3 probe in cones of wild type (WT) and nrc^a14 5 days post-fertilization larvae. A: In WT photoreceptors, autolysosomes (magenta-only puncta) were visible (top panel). A: Fewer magenta-only puncta were visible in nrc^a14 cones (bottom panel), indicating that the LC3 positive autophagosomes had not fused with an acidic compartment. B: Shows enlargement of areas shown in boxes in A. White arrow heads indicate GFP+, mCherry+ autophagosomes, magenta arrowheads point to GFP-, mCherry+ autolysosomes. Scale bar = 2 µm in A. Graph in C shows average number of autophagosomes (GFP+, mCherry+) and autolysosomes (GFP-, mCherry+) per 20 µm³. Error bars are SEM and n = 7 WT larvae and six nrc^a14 larvae. (***p-value < 0.001, ^*p-value < 0.05 respectively as assessed by Mann-Whitney test).

Acknowledgments

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