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Fig. 6
Construct expression and phenotypes of GAL4-positive and -negative embryos of Rac transgenics. Transgenic F1s were outcrossed to hsp70l:gal4 zebrafish, and cmlc2:egfp+ embryos were heat-shocked at ~26 hpf and imaged at ~50 hpf. Gross morphology, transgene expression, and lens structure of hsp70l:gal4-positive and -negative (A) Rac1WT, (B) Rac1CA, and (C) Rac1DN embryos. Transgene expression was strong and ubiquitous following heat shock, and cryosections reveal lens fiber organization defects in RacCA and RacDN induced transgenics. Scale bar = 25µm.
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