ZFIN Image: Hanovice et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Antibody validation of transgene expression. A: Myc colocalization with mCherry expression in heat-shocked hsp70l:gal4;uas:mCherry-F2A-myc-Cdc42^WT embryos. B: No signal for mCherry or myc was detected in uas:mCherry-F2A-myc-Cdc42^WT transgenics missing the hsp70l:gal4 transgene. C: Rac1 antibody staining in ptf1a:gal4;uas:gfp-f2a-Rac1^WT embryos. D: Myc colocalization with mCherry expression in heat-shocked hsp70l:gal4;uas:mCherry-F2A-myc-RhoA^WT retinae. E: No signal for mCherry or myc was detected in uas:mCherry-F2A-myc-RhoA^WT transgenics missing the hsp70l:gal4 transgene. Scale bar = 10µm.

Acknowledgments

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