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Fig. S1
Gnrh3 protein not detectable by another primary antibody in gnrh3-/- adult brains.
(A) Immunohistochemistry on adult coronal brain sections (Aa) using anti-zebrafish Gnrh3 decapeptide (red) demonstrates the presence of Gnrh3 signal in the gnrh3+/+ pre-optic area of the brain (Ab). However, no Gnrh3 signal was found in the gnrh3-/- pre-optic area (Ac) or in any other region of the brain. Scale bars = 100 µm.
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