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Fig. S2
Attempted rescue of Fer MO and overexpression of Fer. Embryos were injected at the 1-cell stage with 2.5 ng Fer MO e9i9 either alone or in combination with increasing amounts of synthetic Fer mRNA (5 ng, 25 ng, 37.5 ng, 50 ng, 100 ng, 150 ng, 200 ng, 250 ng). Co-injection of Fer mRNA did not rescue the Fer knockdown phenotype. B. embryos were injected at the 1-cell stage with Fer WT mRNA. Injection of Fer mRNA by itself induced craniofacial defects, shorter length and heart edema.
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