ZFIN Image: Dworkin et al., 2014, Fig. S2

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Figure Caption

Fig. S2

A grhl3 exon1-intron1 splice-blocking reproduces the edn1-pathway down-regulation seen following use of the ATG-blocking MO. (A-B) Brightfield microscopy showing defective lower jaw development in grhl3-splice-morphants (A) compared to controls (B) (C-F) Alcian blue staining highlighting defective cartilage patterning, showing lateral (C-D) and ventral (E-F) views of grhl3- splice-morphants. (G-N) Expression of pharyngeal markers at 24hpf following injection of the grhl3 splice-blocking MO, showing dlx2in the dorsal arches is unchanged (G, H), whereas the expression of edn1 (I, J), dlx3 (K, L) and hand2 (M, N) is greatly reduced in grhl3-splice-morphants.

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Reprinted from Mechanisms of Development, 133, Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M., Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton, 77-90, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.