ZFIN Image: Dworkin et al., 2014, Fig. S1

Image

Figure Caption

Fig. S1

A grhl3 Exon1/Intron1 splice-blocking morpholino confirms specific targeting of grhl3. (A) Schematic of PCR-primers used to detect non-spliced reverse-transcribed mRNA product following block of grhl3-splicing. (B) The non-spliced grhl3 product was detectable by RT-PCR; this product was absent in MO:control injected fish. (C) The craniofacial phenotype seen following grhl3- knockdown was largely rescued by re-injection of morphants (at the 2-4 cell stage) with grhl3 mRNA, confirming target-specificity; ep, ethmoid plate; hs, hyosymplectic; mc, Meckel’s cartilage; pq, palatoquadrate; ch, ceratohyal; cb1-5, ceratobranchials 1-5.

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Reprinted from Mechanisms of Development, 133, Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M., Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton, 77-90, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.