Fig. 7
The decreased HC regeneration after BIX01294 treatment following neomycin damage can be recued with the addition of bFGF and OF 6-bromoindirubin-3-oxime (BIO). (A) Co-treatment of bFGF and BIO with BIX01294 after neomycin damage increases the number of HCs (Myosin-VI+, red) compared to the BIX01294-only treatment. Nuclei are stained with DAPI. Scale bars = 10 µm. (B) The average number of Myosin-VI+ cells per NM in larvae treated with or without BIX01294 for 48 h after neomycin damage for 1 h and the average number of Myosin-VI+ cells per NM after the addition of bFGF and BIO to controls or BIX01294-treated larvae. Bars are mean ± SEM, and n = total number of embryos. **p < 0.001.