ZFIN Image: Tang et al., 2016, Fig. 4

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Fig. 4

Inhibition of G9a/GLP with UNC0638 also decreases the regeneration of HCs in lateral line NMs. (A) We treated 5 dpf Tg (brn3c:mGFP) zebrafish with neomycin for 1 h and then treated them for 24 h or 48 h with 10 µM UNC0638. HCs in these fish are labeled with anti-GFP antibody (green), and nuclei are stained with DAPI (blue). Scale bars = 10 µm. (B) The average number of GFP⁺ cells per NM in larvae treated with or without 10 µM UNC0638 for 24 h or 48 h after neomycin damage for 1 h. Bars are mean ± SEM, and n = total number of embryos. **p < 0.001. (C) The functional HCs are stained with FM1-43FX (red). Nuclei are stained with DAPI (blue). Scale bars = 10 µm. (D) The average number of FM1-43FX⁺ cells per NM in larvae treated with or without 10 µM UNC0638 for 24 h or 48 h after neomycin damage. Bars are mean ± SEM, and n = total number of embryos. **p < 0.001. (E) HCs in the lateral line NMs were stained with Myosin-VI (red). Nuclei are stained with DAPI. Scale bars = 10 µm. (F) The average number of Myosin-VI⁺ cells per NM in larvae treated with or without 10 µM UNC0638 for 24 h or 48 h after neomycin damage for 1 h. Bars are mean ± SEM, and n = total number of embryos. **p < 0.001.

Acknowledgments

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