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Fig. S4
Neuromuscular junction analysis of Cavin4b/Murcb deficient zebrafish.
Representative confocal maximal projections of whole mount Synaptic Vesicle glycoprotein 2 (SV2, left) and α-bungarotoxin (α-BTX, right) staining of the trunk of 80 hpf murcbs983/+ and murcbs983/s983 zebrafish. SV2 immunofluorescence was used to visualize presynaptic structures; α-BTX staining was used to visualize postsynaptic structures. Synaptic punctae per somite were quantified using ImageJ (dotplot, bottom).
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