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Fig. S1
Morpholinos (MOs) targeting gnb1 (a and b) and gnb4 (a and b) efficiently block translation of the corresponding Gβ proteins, at stages during which the pLLP migrates. (A) Western blot analysis showing the expression of Gb in the indicated embryos at 24 hpf. α-Tubulin was used as a loading control. (B-C) Confocal images of the pLLP showing the expression of Gβ1 (B) and total Gβ (C), in 32-hpf Tg(-8.0cldnb:lynEGFP) embryos with or without injection of the indicated MOs.
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Reprinted from Developmental Biology, 385(2), Xu, H., Ye, D., Behra, M., Burgess, S., Chen, S., and Lin, F., Gbeta1 controls collective cell migration by regulating the protrusive activity of leader cells in the posterior lateral line primordium, 316-27, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.