Fig. S3
Individual reticulospinal axons (A) are of similar caliber when expressing control TdTomato (top) alone or TeNT-TdTomato (second from top) at 5 dpf. Scale bar: 15µm. Areas outlined by boxes in top and second panels are shown imaged by Airyscan in 3rd and 4th panels respectively. Scale bar: 5µm. Individual CoPA axons (B) are of similar caliber when expressing control TdTomato (top) alone or TeNT-TdTomato (second from top) at 5 dpf. Scale bar: 20µm. Areas outlined by boxes in top and second panels are shown imaged by Airyscan in 3rd and 4th panels respectively. Scale bar: 5µm. C and C′ show quantification of axonal caliber and reveal no difference between control and TeNT expressing reticulospinal neurons (C) or CoPA (C′).