Fig. 2
Gene expression and the effect of knocking down genes associated with appetite regulation.
(A) Analysis by qPCR of brain-derived neurotrophic factor mRNA (bdnf) during paramecia feeding, n = 5, *P<0.05. (B and C) Western blots showing embryonic injection of neuropeptide Y (npy) MO (B) and preproinsulin a (insa) MO. (D) Embryonic injection of npy MO and npy mRNA. Orexigenic gene knockdown decreased feeding volume. (E) insa MO and insa mRNA, orexigenic gene knockdown increased feeding volume at 7 dpf. n = 15, *P<0.05. All values are mean ± SEM. (F and G) Knockdown of npy (F) and insa (G) affected locomotor activities at 5 dpf. n = 16, *P<0.05. All values are mean ± SEM. (H) Detection of gene knockdown for splicing MOs in 2% (w/v) agarose gels stained with ethidium bromide.