ZFIN Image: Choorapoikayil et al., 2012, Fig. 4

Image

Figure Caption

Fig. 4

Elevated Akt/PKB signaling in Pten mutant tumors. Immunohistochemistry on transversal sections from a ptena^+/-ptenb^-/- mutant fish, using pAkt- and pGSK-3β-specific antibodies. (A) Tumor area stains weakly positive for pAkt, whereas (B) control vessel (arrow) from the same section is not stained (n=10). (C) Tumor area is highly positive for pGSK-3β, whereas (D) cells from a control vessel (arrow) from the same section only stain mildly positive (n=6). (E) The cranial part of a ptena^+/-ptenb^-/- mutant that developed a tumor was dissected into two fragments, one harboring tumor tissue (tumor) and the other representing control tissue (control). The samples were lysed and the lysates were run on a denaturing SDS-polyacrylamide gel. The proteins were transferred to a PVDF membrane and after blocking the blot was probed with anti-pAkt antibody, stripped and sequentially probed with anti-Akt, anti-pGSK-3, anti-PTEN and, as a loading control, anti-actin.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.