ZFIN Image: Steed et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Cell movements and proliferation may contribute to increased AVC cell density at 48hpf.

(A) Kaede-expressing cells in the atrium, ventricle, or both at 36hpf were photoconverted (photoconverted = magenta; non-photoconverted = green) and the hearts were allowed to develop normally until 48hpf. (B) Imaging stopped hearts at 48hpf showed photoconverted cells of the atrium to be in the AVC at 48hpf. Cells of the ventricle are present in the ventricular inner curvature at 48hpf. (E) Incubation of embryos with BrdU between 36hpf and 48hpf followed by anti-BrdU immunofluorescence analysis demonstrated the presence of BrdU-positive cells (green) in both chambers of the heart. (F) Quantification of BrdU-positive cells in the ventricle, AVC and atrium (n=8). (G) Representation of the movement of cells from the atrium (purple arrow) and the ventricle (blue arrow) between 36hpf and 48hpf. The yellow region represents cells from the AVC of the 36hpf heart, which cluster together on the ventricular side of the AVC at 48hpf. Error bar represents the standard deviation. Scale bars: 10 µm.

Acknowledgments

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