ZFIN Image: Steed et al., 2016, Fig. S1

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Figure Caption

Fig. S1

Contribution of atrial and ventricular cells to the AV valve leaflets at later stages.

Endocardial cells of the atrium or ventricle were photoconverted at 48hpf as shown, and then allowed to develop normally. Hearts were briefly stopped for imaging at 120hpf and 168hpf. No mixing of atrial and ventricular cells was observed in the superior valve leaflet at these later stages. Atrial cells lined the atrial side of the valve leaflet at 120hpf and 168hpf, and ventricular cells lined the ventricular side. Images shown are from the same hearts at 48hpf, 120hpf and 168hpf. White-dotted lines mark the cells containing photoconverted kaede (magenta). Green-dotted lines mark the cells containing nonphotoconverted kaede (green). Scale bars: 10 µm.

Acknowledgments

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