ZFIN Image: Enyedi et al., 2016, Fig. S5

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Figure Caption

Fig. S5

Cell Permeabilization Triggers INM Translocation of cPla₂-mK2 in Zebrafish Larvae Irrespective of Bathing Osmolarity, Related to Figure 5

Left panel, confocal imaging of cPla₂-mK2 localization and nuclear Ca²⁺ levels with GCaMP6s before and after digitonin-induced permeabilization of zebrafish larvae, bathed in isotonic medium. Note the translocation of cPla₂ and the increase in [Ca²⁺] after permeabilization. Right panel, quantification of cPla₂-mK2 INM translocation and average GCaMP6s fluorescence before and after permeabilization in the selected nuclei marked by rainbow colored masks (A-D). Scale bars, 10 µm.

Acknowledgments

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Reprinted from Cell, 165, Enyedi, B., Jelcic, M., Niethammer, P., The Cell Nucleus Serves as a Mechanotransducer of Tissue Damage-Induced Inflammation, 1160-1170, Copyright (2016) with permission from Elsevier. Full text @ Cell