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Fig. 4
The Flk1in10 enhancer requires ETS and GATA motifs for endothelial expression. A and B, Summary of reporter gene expression detected in (A) 48 hpf Tol2-mediated mosaic transient transgenic zebrafish embryos and (B) E12 transient transgenic mice. Asterisk indicates weak staining in head region only. C-F, Whole-mount (top) and transverse sections (bottom) of representative E12 X-gal-stained embryos transgenic for the Flk1in10mutGATA-b,c construct. Depicted sections correspond to the embryos shown. Number in bottom corner of whole-mount embryo denotes the number of embryos similar to that shown. Lines marked i and ii on C mark the approximate location of transverse sections. Arrowheads mark head vessels. G, Analysis of scrambled, 1.125 ng, 2.25 ng, and 3.375 ng gata2a morpholino (MO) in 48 h post fertilization (hpf) tg(Flk1in10:GFP) embryos. H, Analysis of scrambled, 2.25 ng and 3.375 ng gata2a MO in 26 hpf WT zebrafish embryos, using whole-mount kdr in situ hybridization. cv indicates cardinal vein; da, doral aorta; and GFP, green fluorescent protein.