ZFIN Image: Smith et al., 2016, Fig. 7

Image

Figure Caption

Fig. 7

Ectopically located MEP glia do not elicit an immune response. A: Images of control and MTZ-treated Tg(mpeg:egfp);Tg(gfap:nsfB-mcherry) and Tg(pU1:egfp);Tg(gfap:nsfB-mcherry) larvae at 3 dpf. Arrowheads denote macrophages outside of the spinal cord. Arrow denotes microglia inside the spinal cord. Dashed lines denote the edge of the spinal cord. B: Transverse sections through Tg(pU1:egfp) embryos that were fixed at 5 dpf and stained with an antibody specific to acetylated tubulin. Arrow denotes location of microglia. C: Images of a Tg(pU1:egfp);Tg(gfap:nsfB-mcherry) embryo treated with MTZ at 24 hpf and imaged at 5 dpf. Arrows denote microglia. Note the lack of mcherry⁺ debris. D: Quantification of the location of microglia and ectopically located MEP glia within the spinal cord in MTZ-treated embryos at 3 and 5 dpf. Scale bars, 25 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Glia