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Fig. 3
Whole-mount immunofluorescence staining of the 30 dpf zebrafish gut using antibody against galanin (a-c), neuronal marker Hu (a), and acetylated tubulin (b, c). Arrows point to galaninergic neurons in the autonomic ganglion, and the arrowhead shows a very dense network of galanin-IR fibers in the primary islet of the endocrine pancreas. The secondary islets (hollow arrows, c), the vagus nerve (hollow arrowheads, b, c) and its associated galanin-IR neurons are also visible. Dashed line indicates the pancreas (a, c). I intestine, L liver. Scale bars 100 µm
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Full text @ Histochem. Cell Biol.