Fig. S3
Requirement for HSP70 or HSP90 in zebrafish blood vessel development. (A) Fli1-GFP zebrafish embryos injected with control morpholinos or with morpholinos specific for HSP70 or HSP90β followed by vasculature staining for GFP. The results shown are representative of two experiments in each of which at least 100 embryos were injected with each morpholino. CA, Caudal Artery; CV, Caudal Vein; DLAV, Dorsal Longitudinal Anastomotic Vessel; Se, Intersegmental Vessel; DA, Dorsal Aorta; PCV, Posterior Cardinal Vein. (B) HSP70 or (C) HSP90β protein levels determined by immunoblot analysis; tubulin served as a control.