ZFIN Image: Zhao et al., 2016, Fig. 7

Image

Figure Caption

Fig. 7

Domains of Grk2 required for Hh pathway activity

A. Schematic representation of the zebrafish Grk2 protein showing the location of residues assayed for the effects of their mutation on rescuing activity.

B. Prox1a (green) and Eng (red) expression in the myotome of 30hpf MZgrk2 embryos that were injected with mRNA encoding: Grk2^K220RGFP; Grk2^K220MGFP; Grk2^D335NGFP; Grk2^ΔN7aaGFP; and mGrk2^L4AV7AL8AGFP. Merged panels show nuclear staining with DAPI in blue (n = 20 for each sample). Scale bar, 50 µm.

C. Hh reporter activity assay in wildtype and Grk2^-/- 3T3 cells transfected with the equivalent wildtype and mutant kinase domain forms of mouse Grk2, Grk2^K220R, Grk2^K220M and Grk2^D335N after 24h ShhN and SAG treatment. Data represent the mean and ± SD (n = 3). Unpaired Student′s ttest was used for analysis. ****P < 0.0001; **P < 0.01; *P < 0.05; and n.s. (not significant).

Acknowledgments

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