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Fig. 4 S1
mespaa and mespab are Nodal target genes and Nodal ligand expression is not affected in aplnra/b morphant embryos.
(A) Animal view of mespab expression at 50% epiboly (5.25 hpf) in embryos treated with 10 µM of SB505124 from 4-5.25 hpf. Animal cap view with dorsal to the bottom. (B) Animal view of mespab expression at 50% epiboly (5.25 hpf) in embryos in which cells expressing 4 pg of sqt RNA were transplanted into the animal cap. Animal cap view with dorsal to the bottom. (C-N) Lateral view of the Nodal ligands sqt (C-H) and cyc (I-N) expression at sphere (4 hpf), 30% epiboly (4.7 hpf) and 50% epiboly (5.25 hpf) in WT and aplnra/b morphant embryos.