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Fig. 8
The aura mutant phenotype is enhanced by actin polymerization inhibitor and ameliorated by actin stabilizer. In wild type, cytochalasin D leads to a lack of F-actin arcs (B,B′; 17/17) and phalloidin does not have an effect (C,C′; 23/23). In aura mutant embryos, cytochalasin D leads to strongly punctate F-actin (G,G′; 17/17), and phalloidin reverses the phenotype, stabilizing F-actin arcs (H,H′, arrows; 11/11). Microtubule inhibiting (nocodazole, D,D′,I,I′) and stabilizing (taxol, E,E′,J,J′) drugs do not change the dynamic F-actin reorganization in wild-type (16/16 and 15/15, respectively) or aura mutant (15/15 and 10/10, respectively) embryos. (A′-J′) Higher magnifications of boxed regions in A-J. Embryos are at 35mpf. Scale bars: 10µm.