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Fig. 6
Mutation of PG synthesis has no significant effect on total elemental content. This figure compares the elemental levels and distribution in cartilage from wild-type (A-F) and fam20b- mutant (G-L) fish. After quantitation (M), there were no differences in total sulfur [or any other element (P, Cl, K, and Ca)] from data collected at 3 µm steps, in a 5 µm × 7 µm beam. A Student?s t test at the 95% confidence limit was used to test for significant differences between groups. The scale bar is 5 µm. XFI elemental maps were collected at the CLS. The data are presented as relative concentration (fluorescence yield).