ZFIN Image: Ohnmacht et al., 2016, Fig. 8

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Figure Caption

Fig. 8

Motor neuron ablation leads to regeneration of motor neurons. (A) Experimental timeline. (B-H) In spinal cross sections, Hb9⁺/EdU⁺ motor neurons are only observed in MTZ-treated larvae (B,C, shown in a whole cross section of the spinal cord including orthogonal views in F), whereas Pax2⁺ interneurons are labelled by EdU in untreated and MTZ-treated larvae, quantified in G (Mann–Whitney U-test, **P=0.0063) and H (Mann–Whitney U-test, P>0.99), respectively. (I) Overall numbers of Hb9⁺ and ChAT⁺ profiles are reduced after a lesion, but return to control values at 7dpf (Hb9 only) or 10dpf (ChAT) (t-test, *P=0.0188; **P=0.0012; ***P=0.0004). Values are means±s.e.m. Scale bars: 25µm in B for B-E; 5µm in inset in C for all insets; 25µm in F.

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