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Fig. 1
A mechanical lesion to the spinal cord heals within 48h and leads to motor neuron regeneration close to the lesion site. (A) Time line for experiments. (B) A zebrafish larva with a lesion in the dorsal trunk area, leaving the notochord intact at 3dpf. (C,D) The same larva imaged at 0 and 48h post lesion shows closure of the wound. (E,F) The lesion area is outlined with double-labelled Hb9:GFP+/EdU+ neurons indicated by arrowheads. A lesion leads to increased numbers of Hb9:GFP+/EdU+ double-labelled motor neurons (compare E and F). E′ to F′′′ show higher magnifications of areas boxed in E and F, respectively, in single optical sections indicating double labelling. (G) Motor neurons are regenerated close to (<50µm rostral and caudal), but not far from (100µm>×>50µm rostral and caudal) the lesion site (one-way ANOVA with Bonferroni′s multiple comparisons test, ***P<0.001, ****P<0.0001; unlesioned, n=10; far, n=11; close, n=11). (H) Time line of the increase in the number of EdU-labelled motor neurons (t-test, ****P<0.0001). Values are means ± s.e.m. Lateral views of larvae are shown (rostral is left; dorsal is up). Scale bars: 100µm in B; 500µm in D for C,D; 50µm in F for E,F; 15µm in F′′′ for E′-F′′′.