Fig. 3
Effects of Caffeine on hepatic steatosis and lipid contents in overfed zebrafish larvae for 20 days. a The effects of caffeine on weight in zebrafish larvae overfed for 20 days. n = 2 clutches per group, n = 79-86 per clutch. b The food intake of 15 larvae in normal feeding and 5 % caffeine feeding group (n = 4). c Hepatic steatosis rate in zebrafish larvae overfed for 20 days (n = 3). The contents of triglyceride (d) and total cholesterol (e) were measured in zebrafish larvae overfed with different concentration of caffeine for 20 days (n = 3). Data is expressed as mean ± SEM. *P < 0.05, **P < 0.001, ***P < 0.001 by one-way ANOVA