Fig. S9

Fig. S9

The rescue and phenocopy of mibⁿⁿ²⁰⁰² phenotypes. In rescue experiments, her4 WISH was carried out in the mib mRNA (1 ng/µl) injected mib^ta52b and mibⁿⁿ²⁰⁰² embryos. 19 hpf embryos in lateral views with head toward left were shown. The expression of her4 was rescued (increased) by mib mRNA in mib^ta52b and mibⁿⁿ²⁰⁰² homozygotes. huc WISH was carried out in the mib2 mRNA (1 ng/µl) injected mib^ta52b and mib mRNA (1 ng/µl) injected mibⁿⁿ²⁰⁰² embryos. 19 hpf embryos in flat mount with head toward top were shown. huc was rescued (decreased) by mib2 mRNA in mib^ta52b and mib mRNA in mibⁿⁿ²⁰⁰² homozygotes. In phenocopy experiments, mib morpholino injected-wt and -mibⁿⁿ²⁰⁰² embryos were examined. Morpholino can successfully convert 36% of wt and 27% mibⁿⁿ²⁰⁰² siblings into embryos that exhibit typical mib phenotypes (^ta52blike). However, the morpholino failed to convert the mibⁿⁿ²⁰⁰² homozygous embryos into embryos having typical mib phenotypes. The ratio of embryos carried mibⁿⁿ²⁰⁰² phenotype is 21% without injection and 26% with injection. The ratio of embryos that exhibited mibⁿⁿ²⁰⁰² phenotypes was not reduced with the injection of mib morpholino.