IMAGE

Fig. 4

ID
ZDB-IMAGE-160513-6
Publication
Andrews et al., 2016 - Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time
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Figures for Andrews et al., 2016
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Figure Caption

Fig. 4

Irradiation causes an increase in fluorescence lifetime at 3.5 hours in the head region of zebrafish. 24 hpf Tg(Ubi : Caspase3bios) were irradiated (C-F) with 25 Gy from a 137Cs source, or left untreated (A-C) and imaged at 1 hpi (A, D), 2 hpi (B, E) and 3.5 hpi (C, F) with FLIM OPT. 5 time gated images were acquired every 4°, through a full 360° rotation. Data was reconstructed using MATLAB, and fitted using a single pixelwise variable projection model in FLIMfit, giving the mean lifetime per pixel. 3D images were generated using the 3D viewer plug-in in FiJi. Histograms show frequency of pixel lifetimes. Centre histograms: head region. Edge histograms: tail region.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biophotonics
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