Fig. 4

Fig. 4

kif3c function in ciliogenesis. (A and A′) Confocal images of transverse cryosections through the retina stained with Zpr-1 (to visualize double cones; green), and anti-green opsin antibodies (red). (B and B′) Confocal images of lateral cristae in whole animals stained with anti-acetylated tubulin antibody to visualize kinocilia (green) and counterstained with phalloidin (red). WT and kif3b^jj203-/-;kif17^sa0119-/- double mutant animals were analyzed as indicated. (C) Cilia length in WT, mutant, and double mutant cristae as indicated. The average WT length is set at 100%. “n” is the number of individuals analyzed. (D) A schematic drawing of the exon/intron structure for the kif3c and kif3c-like genes. Morpholino-targeted exons are in color. Target sites are indicated as red horizontal bars. (E) Confocal images of lateral cristae in whole animals stained as in B. The number of individuals tested that differentiate cilia is indicated in lower left corner of each panel. (F) Confocal images of transverse cryosections through the retina stained with an anti-acetylated tubulin antibody to visualize cilia (arrows). For E and F, genotypes are indicated below. Brackets indicate the photoreceptor cell layer, arrowheads the outer limiting membrane. (G) The frequency of photoreceptor cilia at 4 dpf in different mutant/morphant backgrounds as indicated. (H) The frequency of photoreceptor cilia in kif3b^-/- mutant homozygotes at 3 dpf, following rescue with kif3c or control GFP mRNA. In G and H, each dot represents the number of cilia per an arbitrary segment of the photoreceptor cell layer in a single retina. (I and I′) Confocal images of photoreceptor cilia (arrows) at 3 dpf visualized as in F. (J) Relative sizes of apical opsin-positive compartments (presumably outer segments) in kif3b^jj203 homozygous mutant and phenotypically WT animals treated with kif3c or control GFP mRNA. Each dot represents the total size of apical opsin-positive domains on a single section through the retina, adjusted for the length of the photoreceptor cell layer. The average WT size equals 100%. (K) Efficiency of GFP–opsin transport in kif3c morphants measured as in Fig. S1. “n” is the number of photoreceptors analyzed. A minimum of 30 sections from six retinae were used to calculate each data point. In G, H, and J, “n” is the number of retinae analyzed. In all images, apical direction is up (*P < 0.05; **P < 0.001).