ZFIN Image: Felker et al., 2016, Fig. 4

Image

Figure Caption

Fig. 4

In contrast to Endoxifen, trans-4-OHT loses its potency over time independently from isomerization. (A) Switching efficiency was quantified comparing fresh and 12 weeks old trans-4-OHT and Endoxifen (scale bar 500 �m). (B) mCherry intensity in embryos treated with 12 weeks old trans-4-OHT is approximately half compared to intensities measured in embryos induced with fresh trans-4-OHT (two-tailed, unpaired t-test, p<0.0001). The potency of Endoxifen does not change significantly throughout the same time period (two-tailed, unpaired t-test, p = 4598). (C) The ¹H NMR spectrum of trans-4-OHT freshly dissolved in DMSO-d6 reveals the presence of ≥98% of the trans isomer. (D) After 5 months storage in DMSO-d6, no appreciable change in isomer composition can be detected by ¹H NMR.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One