|
Fig. S1
Relative CTCF of embryos treated with trans-4-OHT stored at 5 mM. (A) Relative CTCF of ubi:creERT2; ubi:Switch embryos treated with fresh trans-4-OHT compared to treatment with aged trans-4-OHT stored in 5 mM Ethanol, either unheated or heated to 65°C. To compare the activity between fresh and aged trans-4-OHT, we used a one-way ANOVA (two-tailed, unpaired t-test, aged vs heated p = 0.0126). (B-E) Lateral confocal images of mCherry expression in the anterior trunk of larvae untreated (B) or treated with fresh trans-4-OHT (C), aged trans-4-OHT dissolved in 5 mM Ethanol and stored for two years (D) and aged trans-4-OHT dissolved in 5 mM Ethanol and stored for two years and then heated at 65°C.